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Journal of Biomedical Optics • Open Access

Multifocus confocal Raman microspectroscopy for rapid single-particle analysis
Author(s): Lingbo Kong; Pengfei Zhang; Yong-qing Li; Peter Setlow

Paper Abstract

We have developed a multifocus confocal Raman microspectroscopy system that allows simultaneous analyses of ∼80 individual biological or airborne microparticles based on a precise image-guided technique. Multiple individual particles adhered in random positions on a coverslip were illuminated by a multifocus excitation pattern formed by rapidly steering a single laser beam with a pair of galvo-mirrors, and their Raman scatterings were synchronously projected with another galvo-mirror to different rows of a CCD chip for parallel spectroscopic analyses. We show that this technique can be used to rapidly identify single airborne particles or bacteria collected on a slide and to monitor germination dynamics of multiple bacterial spores in real-time.

Paper Details

Date Published: 1 December 2011
PDF: 4 pages
J. Biomed. Opt. 16(12) 120503 doi: 10.1117/1.3662456
Published in: Journal of Biomedical Optics Volume 16, Issue 12
Show Author Affiliations
Lingbo Kong, East Carolina Univ. (United States)
Pengfei Zhang, East Carolina Univ. (United States)
Yong-qing Li, East Carolina Univ. (United States)
Peter Setlow, Univ. of Connecticut Health Ctr. (United States)

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