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Journal of Biomedical Optics • Open Access

Low-cost three-dimensional imaging system combining fluorescence and ultrasound
Author(s): Baoqiang Li; Maxime Abran; Carl Matteau-Pelletier; Leonie Rouleau; Frédéric Lesage; Eric Rheaume; Jean-Claude Tardif; Tina Lam; Rishi Sharma; Ashok Kakkar

Paper Abstract

In this paper, we present a dual-modality imaging system combining three-dimensional (3D) continuous-wave transillumination fluorescence tomography with 3D ultrasound (US) imaging. We validated the system with two phantoms, one containing fluorescent inclusions (Cy5.5) at different depths, and another varying-thickness semicylindrical phantom. Using raster scanning, the combined fluorescence/US system was used to collect the boundary fluorescent emission in the X-Y plane, as well as recovered the 3D surface and position of the inclusions from US signals. US images were segmented to provide soft priors for the fluorescence image reconstruction. Phantom results demonstrated that with priors derived from the US images, the fluorescent reconstruction quality was significantly improved. As further evaluation, we show pilot in vivo results using an Apo-E mouse to assess the feasibility and performance of this system in animal studies. Limitations and potential to be used in artherosclerosis studies are then discussed.

Paper Details

Date Published: 1 December 2011
PDF: 11 pages
J. Biomed. Opt. 16(12) 126010 doi: 10.1117/1.3662455
Published in: Journal of Biomedical Optics Volume 16, Issue 12
Show Author Affiliations
Baoqiang Li, Ecole Polytechnique de Montréal (Canada)
Maxime Abran, Ecole Polytechnique de Montréal (Canada)
Carl Matteau-Pelletier, Ecole Polytechnique de Montréal (Canada)
Leonie Rouleau, Ecole Polytechnique de Montréal (Canada)
Frédéric Lesage, Ecole Polytechnique de Montréal (Canada)
Eric Rheaume, Montréal Heart Institute (Canada)
Jean-Claude Tardif, Montréal Heart Institute (Canada)
Tina Lam, McGill Univ. (Canada)
Rishi Sharma, McGill Univ. (Canada)
Ashok Kakkar, McGill Univ. (Canada)

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