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Journal of Biomedical Optics

Lensless multispectral digital in-line holographic microscope
Author(s): James P. Ryle; John T. Sheridan; Susan McDonnell
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Paper Abstract

An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.

Paper Details

Date Published: 1 December 2011
PDF: 18 pages
J. Biomed. Opt. 16(12) 126004 doi: 10.1117/1.3659681
Published in: Journal of Biomedical Optics Volume 16, Issue 12
Show Author Affiliations
James P. Ryle, Univ. College Dublin (Ireland)
John T. Sheridan, Univ. College Dublin (Ireland)
Susan McDonnell, Univ. College Dublin (Ireland)

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