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Journal of Biomedical Optics

Discriminating adenocarcinoma from normal colonic mucosa through deconvolution of Raman spectra
Author(s): Patricia C. Lopes; Joaquim A. Moreira; Abílio Almeida; Artur Esteves; Ivan Gregora; Martin Ledinsky; Jose M. Lopes; Rui Henrique; Albino Oliveira
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Paper Abstract

In this work, we considered the feasibility of Raman spectroscopy for discriminating between adenocarcinomatous and normal mucosal formalin-fixed colonic tissues. Unlike earlier studies in colorectal cancer, a spectral deconvolution model was implemented to derive spectral information. Eleven samples of human colon were used, and 55 spectra were analyzed. Each spectrum was resolved into 25 bands from 975 to 1720 cm−1, where modes of proteins, lipids, and nucleic acids are observed. From a comparative study of band intensities, those presenting higher differences between tissue types were correlated to biochemical assignments. Results from fitting procedure were further used as inputs for linear discriminant analysis, where combinations of band intensities and intensity ratios were tested, yielding accuracies up to 81%. This analysis yields objective discriminating parameters after fitting optimization. The bands with higher diagnosis relevance detected by spectra deconvolution enable to confine the study to some spectral regions instead of broader ranges. A critical view upon limitations of this approach is presented, along with a comparison of our results to earlier ones obtained in fresh colonic tissues. This enabled to assess the effect of formalin fixation in colonic tissues, and determine its relevance in the present analysis.

Paper Details

Date Published: 1 December 2011
PDF: 13 pages
J. Biomed. Opt. 16(12) 127001 doi: 10.1117/1.3658756
Published in: Journal of Biomedical Optics Volume 16, Issue 12
Show Author Affiliations
Patricia C. Lopes, Univ. do Porto (Portugal)
Joaquim A. Moreira, Univ. do Porto (Portugal)
Abílio Almeida, Univ. do Porto (Portugal)
Artur Esteves, Univ. do Porto (Portugal)
Ivan Gregora, Institute of Physics of the ASCR, v.v.i. (Czech Republic)
Martin Ledinsky, Institute of Physics of the ASCR, v.v.i. (Czech Republic)
Jose M. Lopes, Portugese Oncology Institute (Portugal)
Rui Henrique, Portugese Oncology Institute (Portugal)
Albino Oliveira, São Sebastião Hospital (Portugal)

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