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Journal of Biomedical Optics

In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence
Author(s): Regina B. Orzekowsky-Schroeder; Bjoern Martensen; Alfred Vogel; Gereon Hüttmann; Antje Klinger; Maike Blessenohl; Andreas Gebert
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Paper Abstract

Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selective excitation with only two different wavelengths within the tuning range of a Ti:sapphire femtosecond laser system yields excellent discrimination between enterocytes, antigen presenting cells and lysosomes based on the excitation and emission properties of their autofluorescence. The method is employed for time-lapse microscopy over up to 8 h. Changes of the spectral signature with the onset of photodamage are demonstrated, and their origin is discussed.

Paper Details

Date Published: 1 November 2011
PDF: 14 pages
J. Biomed. Opt. 16(11) 116025 doi: 10.1117/1.3655587
Published in: Journal of Biomedical Optics Volume 16, Issue 11
Show Author Affiliations
Regina B. Orzekowsky-Schroeder, Univ. zu Lübeck (Germany)
Bjoern Martensen, Univ. zu Lübeck (Germany)
Alfred Vogel, Univ. zu Lübeck (Germany)
Gereon Hüttmann, Univ. zu Lübeck (Germany)
Antje Klinger, Univ. zu Lübeck (Germany)
Maike Blessenohl, Univ. zu Lübeck (Germany)
Andreas Gebert, Univ. zu Lübeck (Germany)

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