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Journal of Biomedical Optics • Open Access

Assessment of liver steatosis and fibrosis in rats using integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique
Author(s): Jian Lin; Fake Lu; Wei Zheng; Zhiwei Huang; Shuoyu Xu; Dean C. S. Tai; Hanry Yu

Paper Abstract

We report the implementation of a unique integrated coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and two-photon excitation fluorescence (TPEF) microscopy imaging technique developed for label-free monitoring of the progression of liver steatosis and fibrosis generated in a bile duct ligation (BDL) rat model. Among the 21 adult rats used in this study, 18 rats were performed with BDL surgery and sacrificed each week from weeks 1 to 6 (n = 3 per week), respectively; whereas 3 rats as control were sacrificed at week 0. Colocalized imaging of the aggregated hepatic fats, collagen fibrils, and hepatocyte morphologies in liver tissue is realized by using the integrated CARS, SHG, and TPEF technique. The results show that there are significant accumulations of hepatic lipid droplets and collagen fibrils associated with severe hepatocyte necrosis in BDL rat liver as compared to a normal liver tissue. The volume of normal hepatocytes keeps decreasing and the fiber collagen content in BDL rat liver follows a growing trend until week 6; whereas the hepatic fat content reaches a maximum in week 4 and then appears to stop growing in week 6, indicating that liver steatosis and fibrosis induced in a BDL rat liver model may develop at different rates. This work demonstrates that the integrated CARS and multiphoton microscopy imaging technique has the potential to provide an effective means for early diagnosis and detection of liver steatosis and fibrosis without labeling.

Paper Details

Date Published: 1 November 2011
PDF: 8 pages
J. Biomed. Opt. 16(11) 116024 doi: 10.1117/1.3655353
Published in: Journal of Biomedical Optics Volume 16, Issue 11
Show Author Affiliations
Jian Lin, National Univ. of Singapore (Singapore)
Fake Lu, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)
Shuoyu Xu, Singapore-MIT Alliance (Singapore)
Dean C. S. Tai, Institute of Bioengineering and Nanotechnology (Singapore)
Hanry Yu, Institute of Bioengineering and Nanotechnology (Singapore)

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