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Journal of Biomedical Optics • Open Access

Wavelength selection in measuring red blood cell aggregation based on light transmittance
Author(s): Mehmet Uyuklu; Murat Canpolat; Herbert J. Meiselman; Oguz K. Baskurt

Paper Abstract

The reversible aggregation of red blood cells (RBC) is of current basic science and clinical interest. Using a flow channel and light transmittance (LT) through RBC suspensions, we have examined the effects of wavelength (500 to 900 nm) on the static and dynamic aspects of RBC aggregation for normal blood and suspensions with reduced or enhanced aggregation; the effects of oxygenation were also explored. Salient observations include: 1. significant effects of wavelength on aggregation parameters reflecting the extent of aggregation (i.e., number of RBC per aggregate); 2. no significant effects of wavelength on parameters reflecting the time course of RBC aggregation; 3. a prominent influence of hemoglobin oxygen saturation on both extent and time-course related aggregation parameters measured at wavelengths less than 700 nm, but only on the time-course at 800 nm; and 4. the power of parameters in detecting a given alteration of RBC aggregation is affected by wavelength, in general being greater at higher wavelengths. It is recommended that light sources with wavelengths around 800 nm be used in instruments for measuring RBC aggregation via LT.

Paper Details

Date Published: 1 November 2011
PDF: 10 pages
J. Biomed. Opt. 16(11) 117006 doi: 10.1117/1.3652712
Published in: Journal of Biomedical Optics Volume 16, Issue 11
Show Author Affiliations
Mehmet Uyuklu, Akdeniz Üniv. (Turkey)
Murat Canpolat, Akdeniz Üniv. (Turkey)
Herbert J. Meiselman, The Univ. of Southern California (United States)
Oguz K. Baskurt, Koc Univ. School of Medicine (Turkey)

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