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Journal of Biomedical Optics

Evaluating the use of optical coherence tomography for the detection of epithelial cancers in vitro
Author(s): Louise E. Smith; Vanessa Hearnden; Zenghai Lu; Stephen J. Matcher; Sheila MacNeil; Martin Thornhill; Craig Murdoch; Rod H. Smallwood; Keith D. Hunter
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Paper Abstract

Optical coherence tomography (OCT) is a noninvasive imaging methodology that is able to image tissue to depths of over 1 mm. Many epithelial conditions, such as melanoma and oral cancers, require an invasive biopsy for diagnosis. A noninvasive, real-time, point of care method of imaging depth-resolved epithelial structure could greatly improve early diagnosis and long-term monitoring in patients. Here, we have used tissue-engineered (TE) models of normal skin and oral mucosa to generate models of melanoma and oral cancer. We have used these to determine the ability of OCT to image epithelial differences in vitro. We report that while in vivo OCT gives reasonable depth information for both skin and oral mucosa, in vitro the information provided is less detailed but still useful. OCT can provide reassurance on the development of TE models of skin and oral mucosa as they develop in vitro. OCT was able to detect the gross alteration in the epithelium of skin and mucosal models generated with malignant cell lines but was less able to detect alteration in the epithelium of TE models that mimicked oral dysplasia or, in models where tumor cells had penetrated into the dermis.

Paper Details

Date Published: 1 November 2011
PDF: 9 pages
J. Biomed. Opt. 16(11) 116015 doi: 10.1117/1.3652708
Published in: Journal of Biomedical Optics Volume 16, Issue 11
Show Author Affiliations
Louise E. Smith, The Univ. of Sheffield (United Kingdom)
Vanessa Hearnden, The Univ. of Sheffield (United Kingdom)
Zenghai Lu, The Univ. of Sheffield (United Kingdom)
Stephen J. Matcher, The Univ. of Sheffield (United Kingdom)
Sheila MacNeil, The Univ. of Sheffield (United Kingdom)
Martin Thornhill, The Univ. of Sheffield (United Kingdom)
Craig Murdoch, The Univ. of Sheffield (United Kingdom)
Rod H. Smallwood, The Univ. of Sheffield (United Kingdom)
Keith D. Hunter, The Univ. of Sheffield (United Kingdom)


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