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Journal of Biomedical Optics • Open Access

Single myelin fiber imaging in living rodents without labeling by deep optical coherence microscopy
Author(s): Juliette Ben Arous; Jonas Binding; Jean-Francois Leger; Mariano Casado; Piotr Topilko; Laurent Bourdieu; Sylvain Gigan; A. Claude Boccara

Paper Abstract

Myelin sheath disruption is responsible for multiple neuropathies in the central and peripheral nervous system. Myelin imaging has thus become an important diagnosis tool. However, in vivo imaging has been limited to either low-resolution techniques unable to resolve individual fibers or to low-penetration imaging of single fibers, which cannot provide quantitative information about large volumes of tissue, as required for diagnostic purposes. Here, we perform myelin imaging without labeling and at micron-scale resolution with <300-μm penetration depth on living rodents. This was achieved with a prototype [termed deep optical coherence microscopy (deep-OCM)] of a high-numerical aperture infrared full-field optical coherence microscope, which includes aberration correction for the compensation of refractive index mismatch and high-frame-rate interferometric measurements. We were able to measure the density of individual myelinated fibers in the rat cortex over a large volume of gray matter. In the peripheral nervous system, deep-OCM allows, after minor surgery, in situ imaging of single myelinated fibers over a large fraction of the sciatic nerve. This allows quantitative comparison of normal and Krox20 mutant mice, in which myelination in the peripheral nervous system is impaired. This opens promising perspectives for myelin chronic imaging in demyelinating diseases and for minimally invasive medical diagnosis.

Paper Details

Date Published: 1 November 2011
PDF: 10 pages
J. Biomed. Opt. 16(11) 116012 doi: 10.1117/1.3650770
Published in: Journal of Biomedical Optics Volume 16, Issue 11
Show Author Affiliations
Juliette Ben Arous, Ecole Normale Supérieure (France)
Jonas Binding, Ecole Supérieure de Physique et de Chimie Industrielles (France)
Jean-Francois Leger, Ecole Normale Supérieure (France)
Mariano Casado, Ecole Normale Supérieure (France)
Piotr Topilko, Ecole Normale Supérieure (France)
Laurent Bourdieu, Ecole Normale Supérieure (France)
Sylvain Gigan, Ecole Supérieure de Physique et de Chimie Industrielles (France)
A. Claude Boccara, Ecole Supérieure de Physique et de Chimie Industrielles (France)

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