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Journal of Biomedical Optics • Open Access

Raman spectroscopic characterization and differentiation of seminal plasma
Author(s): Zufang Huang; Xiwen Chen; Yanping Chen; Shangyuan Feng; Rong Chen; Jinhua Chen; Min Dou; Haishan Zeng

Paper Abstract

Raman spectroscopy (RS) was applied for the analysis of seminal plasma in order to detect spectral parameters, which might be used for differentiating the normal and abnormal semen samples. Raman spectra of seminal plasma separated from normal and abnormal semen samples, showed a distinct difference in peak ratios between 1449 and 1418 cm−1 (P < 0.05). More efficient alternative method of using principal component analysis-linear discriminate analysis based on Raman spectroscopic data yielded a diagnostic sensitivity of 73% and specificity of 82%. The results suggest that RS combined with the multivariate analysis method has the potential for differentiating semen samples by examination of the corresponding seminal plasma.

Paper Details

Date Published: 1 November 2011
PDF: 4 pages
J. Biomed. Opt. 16(11) 110501 doi: 10.1117/1.3650310
Published in: Journal of Biomedical Optics Volume 16, Issue 11
Show Author Affiliations
Zufang Huang, Fujian Normal Univ. (China)
Xiwen Chen, Fujian Normal Univ. (China)
Yanping Chen, Fujian Normal Univ. (China)
Shangyuan Feng, Fujian Normal Univ. (China)
Rong Chen, Fujian Normal Univ. (China)
Jinhua Chen, Fujian Provincial Hospital (China)
Min Dou, Fujian Provincial Hospital (China)
Haishan Zeng, The BC Cancer Agency Research Ctr. (Canada)

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