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Journal of Biomedical Optics • Open Access

In vivo photoacoustic imaging of chemotherapy-induced apoptosis in squamous cell carcinoma using a near-infrared caspase-9 probe
Author(s): Qiuhong Yang; Shuang Cai; M. Laird Forrest; Huizhong Cui; Xinmai Yang

Paper Abstract

Anti-cancer drugs typically exert their pharmacological effect on tumors by inducing apoptosis, or programmed cell death, within the cancer cells. However, no tools exist in the clinic for detecting apoptosis in real time. Microscopic examination of surgical biopsies and secondary responses, such as morphological changes, are used to verify efficacy of a treatment. Here, we developed a novel near-infrared dye-based imaging probe to directly detect apoptosis with high specificity in cancer cells by utilizing a noninvasive photoacoustic imaging (PAI) technique. Nude mice bearing head and neck tumors received cisplatin chemotherapy (10 mg/kg) and were imaged by PAI after tail vein injection of the contrast agent. In vivo PAI indicated a strong apoptotic response to chemotherapy on the peripheral margins of tumors, whereas untreated controls showed no contrast enhancement by PAI. The apoptotic status of the mouse tumor tissue was verified by immunohistochemical techniques staining for cleaved caspase-3 p11 subunit. The results demonstrated the potential of this imaging probe to guide the evaluation of chemotherapy treatment.

Paper Details

Date Published: 1 November 2011
PDF: 5 pages
J. Biomed. Opt. 16(11) 116026 doi: 10.1117/1.3650240
Published in: Journal of Biomedical Optics Volume 16, Issue 11
Show Author Affiliations
Qiuhong Yang, The Univ. of Kansas (United States)
Shuang Cai, The Univ. of Kansas (United States)
M. Laird Forrest, The Univ. of Kansas (United States)
Huizhong Cui, The Univ. of Kansas (United States)
Xinmai Yang, The Univ. of Kansas (United States)

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