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Journal of Biomedical Optics • Open Access

Estimating the density of fluorescent nanoparticles in the primo vessels in the fourth ventricle and the spinal cord of a rat
Author(s): Jaekwan Lim; Jong Hyun Jung; Sungwoo Lee; Zhendong Su; Zhou Qiang; Kwangsup Soh; Jin-Myung Cha; Jin-Kyu Lee

Paper Abstract

The primo vascular system is a novel circulatory system forming a network throughout an animal's body. Primo vessels were recently observed in the fourth ventricle of the brain and in the spinal cord of a rat by using fluorescent nanoparticles. In order to quantify the nanoparticles in the primo vessels, we measured the florescence of the nanoparticles and calibrated the measurements by using a reference suspension. We removed the noise due to autofluorescence with the technique of multispectral imaging. The line densities of nanoparticles and the contrast values of their images were, respectively, 0.5 ± 0.5 ng/mm and 0.7 ± 0.5 for primo vessels in the fourth ventricle, and 1.3 ± 0.6 ng/mm and 1.4 ± 0.2 for primo vessels in the spinal cord. The data obtained from and the procedures used in this work could be useful in evaluating the feasibility of using nanoparticles as a contrast agent during MRI or CT imaging of primo vessels in the brain or the spinal cord.

Paper Details

Date Published: 1 November 2011
PDF: 8 pages
J. Biomed. Opt. 16(11) 116010 doi: 10.1117/1.3647595
Published in: Journal of Biomedical Optics Volume 16, Issue 11
Show Author Affiliations
Jaekwan Lim, Seoul National Univ. (Korea, Republic of)
Jong Hyun Jung, Seoul National Univ. (Korea, Republic of)
Sungwoo Lee, Seoul National Univ. (Korea, Republic of)
Zhendong Su, Seoul National Univ. (Korea, Republic of)
Zhou Qiang, Seoul National Univ. (Korea, Republic of)
Kwangsup Soh, Seoul National Univ. (Korea, Republic of)
Jin-Myung Cha, Seoul National Univ. (Korea, Republic of)
Jin-Kyu Lee, Seoul National Univ. (Korea, Republic of)


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