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Journal of Biomedical Optics • Open Access

Selective two-photon fluorescence suppression by ultrafast pulse-pair excitation: control by selective one-color stimulated emission

Paper Abstract

Controlling two-photon molecular fluorescence leading to selective fluorophore excitation has been a long sought after goal in fluorescence microscopy. In this letter, we thoroughly explore selective fluorescence suppression through simultaneous two-photon absorption by two different fluorophores followed by selective one-photon stimulated emission for one particular fluorophore. We achieve this by precisely controlling the time delay between two identical ultrafast near infrared laser pulses.

Paper Details

Date Published: 1 October 2011
PDF: 4 pages
J. Biomed. Opt. 16(10) 100505 doi: 10.1117/1.3645082
Published in: Journal of Biomedical Optics Volume 16, Issue 10
Show Author Affiliations
Arijit K. De, Lawrence Berkeley National Lab. (United States)
Debjit Roy, Indian Institute of Technology Kanpur (India)
Debabrata Goswami, Indian Institute of Technology Kanpur (India)

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