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Journal of Biomedical Optics • Open Access

In vivo low-coherence spectroscopic measurements of local hemoglobin absorption spectra in human skin

Paper Abstract

Localized spectroscopic measurements of optical properties are invaluable for diagnostic applications that involve layered tissue structures, but conventional spectroscopic techniques lack exact control over the size and depth of the probed tissue volume. We show that low-coherence spectroscopy (LCS) overcomes these limitations by measuring local attenuation and absorption coefficient spectra in layered phantoms. In addition, we demonstrate the first in vivo LCS measurements of the human epidermis and dermis only. From the measured absorption in two distinct regions of the dermal microcirculation, we determine total hemoglobin concentration (3.0±0.5 g/l and 7.8±1.2 g/l) and oxygen saturation.

Paper Details

Date Published: 1 October 2011
PDF: 4 pages
J. Biomed. Opt. 16(10) 100504 doi: 10.1117/1.3644497
Published in: Journal of Biomedical Optics Volume 16, Issue 10
Show Author Affiliations
Nienke Bosschaart, Academisch Medisch Ctr. (Netherlands)
Dirk J. Faber, Academisch Medisch Ctr. (Netherlands)
Ton G. van Leeuwen, Academisch Medisch Ctr. (Netherlands)
Maurice C. G. Aalders, Academisch Medisch Ctr. (Netherlands)

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