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Journal of Biomedical Optics • Open Access

Quantitative characterization of articular cartilage using Mueller matrix imaging and multiphoton microscopy
Author(s): Pal G. Ellingsen; Magnus B. Lilledahl; Lars Martin S. Aas; Catharina de Lange Davies; Morten Kildemo

Paper Abstract

The collagen meshwork in articular cartilage of chicken knee is characterized using Mueller matrix imaging and multiphoton microscopy. Direction and degree of dispersion of the collagen fibers in the superficial layer are found using a Fourier transform image-analysis technique of the second-harmonic generated image. Mueller matrix images are used to acquire structural data from the intermediate layer of articular cartilage where the collagen fibers are too small to be resolved by optical microscopy, providing a powerful multimodal measurement technique. Furthermore, we show that Mueller matrix imaging provides more information about the tissue compared to standard polarization microscopy. The combination of these techniques can find use in improved diagnosis of diseases in articular cartilage, improved histopathology, and additional information for accurate biomechanical modeling of cartilage.

Paper Details

Date Published: 1 November 2011
PDF: 7 pages
J. Biomed. Opt. 16(11) 116002 doi: 10.1117/1.3643721
Published in: Journal of Biomedical Optics Volume 16, Issue 11
Show Author Affiliations
Pal G. Ellingsen, Norwegian Univ. of Science and Technology (Norway)
Magnus B. Lilledahl, Norwegian Univ. of Science and Technology (Norway)
Lars Martin S. Aas, Norwegian Univ. of Science and Technology (Norway)
Catharina de Lange Davies, Norwegian Univ. of Science and Technology (Norway)
Morten Kildemo, Norwegian Univ. of Science and Technology (Norway)


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