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Journal of Biomedical Optics • Open Access

Multiphoton fluorescence, second harmonic generation, and fluorescence lifetime imaging of whole cleared mouse organs

Paper Abstract

Multiphoton microscopy of cleared tissue has previously been demonstrated to generate large three-dimensional (3D) volumetric image data on entire intact mouse organs using intrinsic tissue fluorescence. This technique holds great promise for performing 3D virtual biopsies, providing unique information on tissue morphology, and guidance for subsequent traditional slicing and staining. Here, we demonstrate the use of fluorescence lifetime imaging in cleared organs for achieving molecular contrast that can reveal morphologically distinct structures, even in the absence of knowledge of the underlying molecular source. In addition, we demonstrate the power of multimodal imaging, combining multiphoton fluorescence, second harmonic generation, and lifetime imaging to reveal exceptional morphological detail in an optically cleared mouse knee.

Paper Details

Date Published: 1 October 2011
PDF: 7 pages
J. Biomed. Opt. 16(10) 106009 doi: 10.1117/1.3641992
Published in: Journal of Biomedical Optics Volume 16, Issue 10
Show Author Affiliations
Sam Vesuna, Yale Univ. (United States)
Richard Torres, Yale School of Medicine (United States)
Michael J. Levene, Yale Univ. (United States)

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