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Journal of Biomedical Optics

Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord
Author(s): Yunzhou Shi; Riyi Shi; Ji-Xin Cheng; Delong Zhang; Terry B. Huff; Xiaofei Wang; Xiao-Ming Xu
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Paper Abstract

In vivo imaging of white matter is important for the mechanistic understanding of demyelination and evaluation of remyelination therapies. Although white matter can be visualized by a strong coherent anti-Stokes Raman scattering (CARS) signal from axonal myelin, in vivo repetitive CARS imaging of the spinal cord remains a challenge due to complexities induced by the laminectomy surgery. We present a careful experimental design that enabled longitudinal CARS imaging of de- and remyelination at single axon level in live rats. In vivo CARS imaging of secretory phospholipase A2 induced myelin vesiculation, macrophage uptake of myelin debris, and spontaneous remyelination by Schwann cells are sequentially monitored over a 3 week period. Longitudinal visualization of de- and remyelination at a single axon level provides a novel platform for rational design of therapies aimed at promoting myelin plasticity and repair.

Paper Details

Date Published: 1 October 2011
PDF: 4 pages
J. Biomed. Opt. 16(10) 106012 doi: 10.1117/1.3641988
Published in: Journal of Biomedical Optics Volume 16, Issue 10
Show Author Affiliations
Yunzhou Shi, Purdue Univ. (United States)
Riyi Shi, Purdue Univ. (United States)
Ji-Xin Cheng, Purdue Univ. (United States)
Delong Zhang, Purdue Univ. (United States)
Terry B. Huff, Purdue Univ. (United States)
Xiaofei Wang, Indiana Univ. School of Medicine (United States)
Xiao-Ming Xu, Purdue Univ. (United States)

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