Share Email Print

Journal of Biomedical Optics • Open Access

Recovery of macular pigment spectrum in vivo using hyperspectral image analysis
Author(s): Amani A. Fawzi; Noah Lee; Andrew F. Laine; Jennifer H. Acton; R. Theodore Smith

Paper Abstract

We investigated the feasibility of a novel method for hyperspectral mapping of macular pigment (MP) in vivo. Six healthy subjects were recruited for noninvasive imaging using a snapshot hyperspectral system. The three-dimensional full spatial-spectral data cube was analyzed using non-negative matrix factorization (NMF), wherein the data was decomposed to give spectral signatures and spatial distribution, in search for the MP absorbance spectrum. The NMF was initialized with the in vitro MP spectrum and rank 4 spectral signature decomposition was used to recover the MP spectrum and optical density in vivo. The recovered MP spectra showed two peaks in the blue spectrum, characteristic of MP, giving a detailed in vivo demonstration of these absorbance peaks. The peak MP optical densities ranged from 0.08 to 0.22 (mean 0.15+/−0.05) and became spatially negligible at diameters 1100 to 1760 μm (4 to 6 deg) in the normal subjects. This objective method was able to exploit prior knowledge (the in vitro MP spectrum) in order to extract an accurate in vivo spectral analysis and full MP spatial profile, while separating the MP spectra from other ocular absorbers. Snapshot hyperspectral imaging in combination with advanced mathematical analysis provides a simple cost-effective approach for MP mapping in vivo.

Paper Details

Date Published: 1 October 2011
PDF: 10 pages
J. Biomed. Opt. 16(10) 106008 doi: 10.1117/1.3640813
Published in: Journal of Biomedical Optics Volume 16, Issue 10
Show Author Affiliations
Amani A. Fawzi, Doheny Eye Institute (United States)
Noah Lee, Columbia Univ. (United States)
Andrew F. Laine, Columbia Univ. (United States)
Jennifer H. Acton, Columbia Univ. (United States)
R. Theodore Smith, Columbia Univ. (United States)

© SPIE. Terms of Use
Back to Top