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Journal of Biomedical Optics

Beyond the lateral resolution limit by phase imaging
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Paper Abstract

We present a theory to extend the classical Abbe resolution limit by introducing a spatially varying phase into the illumination beam of a phase imaging system. It allows measuring lateral and axial distance differences between point sources to a higher accuracy than intensity imaging alone. Various proposals for experimental realization are debated. Concretely, the phase of point scatterers' interference is experimentally visualized by high numerical aperture (NA = 0.93) digital holographic microscopy combined with angular scanning. Proof-of-principle measurements are presented by using sub-wavelength nanometric holes on an opaque metallic film. In this manner, Rayleighs classical two-point resolution condition can be rebuilt. With different illumination phases, enhanced bandpass information content is demonstrated, and its spatial resolution is theoretically shown to be potentially signal-to-noise ratio limited.

Paper Details

Date Published: 1 October 2011
PDF: 7 pages
J. Biomed. Opt. 16(10) 106007 doi: 10.1117/1.3640812
Published in: Journal of Biomedical Optics Volume 16, Issue 10
Show Author Affiliations
Yann J. Cotte, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Muhammed Fatih Toy, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Christian D. Depeursinge, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

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