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Journal of Biomedical Optics • Open Access

In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography
Author(s): Andrea Bassi; Luca Fieramonti; Cosimo D'Andrea; Gianluca Valentini; Marina Mione

Paper Abstract

We introduce flow optical projection tomography, an imaging technique capable of visualizing the vasculature of living specimens in 3-D. The method detects the movement of cells in the bloodstream and creates flow maps using a motion-analysis procedure. Then, flow maps obtained from projection taken at several angles are used to reconstruct sections of the circulatory system of the specimen. We therefore demonstrate an in vivo, 3-D optical imaging technique that, without the use of any labeling, is able to reconstruct and visualize the vascular network of transparent and weakly scattering living specimens.

Paper Details

Date Published: 1 October 2011
PDF: 4 pages
J. Biomed. Opt. 16(10) 100502 doi: 10.1117/1.3640808
Published in: Journal of Biomedical Optics Volume 16, Issue 10
Show Author Affiliations
Andrea Bassi, Politecnico di Milano (Italy)
Luca Fieramonti, Politecnico di Milano (Italy)
Cosimo D'Andrea, Politecnico di Milano (Italy)
Gianluca Valentini, Politecnico di Milano (Italy)
Marina Mione, IFOM-IEO (Italy)

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