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Journal of Biomedical Optics • Open Access

Retinal oximeter for the blue-green oximetry technique
Author(s): Kurt R. Denninghoff; Lawrence DeLuca; Katarzyna Sieluzycka; Jennifer K. Hendryx; Tyson J. Ririe; Russell A. Chipman

Paper Abstract

Retinal oximetry offers potential for noninvasive assessment of central venous oxyhemoglobin saturation (SO2) via the retinal vessels but requires a calibrated accuracy of ±3% saturation in order to be clinically useful. Prior oximeter designs have been hampered by poor saturation calibration accuracy. We demonstrate that the blue-green oximetry (BGO) technique can provide accuracy within ±3% in swine when multiply scattered light from blood within a retinal vessel is isolated. A noninvasive on-axis scanning retinal oximeter (ROx-3) is constructed that generates a multiwavelength image in the range required for BGO. A field stop in the detection pathway is used in conjunction with an anticonfocal bisecting wire to remove specular vessel reflections and isolate multiply backscattered light from the blood column within a retinal vessel. This design is tested on an enucleated swine eye vessel and a retinal vein in a human volunteer with retinal SO2 measurements of ∼1 and ∼65%, respectively. These saturations, calculated using the calibration line from earlier work, are internally consistent with a standard error of the mean of ±2% SO2. The absolute measures are well within the expected saturation range for the site (-1 and 63%). This is the first demonstration of noninvasive on-axis BGO retinal oximetry.

Paper Details

Date Published: 1 October 2011
PDF: 8 pages
J. Biomed. Opt. 16(10) 107004 doi: 10.1117/1.3638134
Published in: Journal of Biomedical Optics Volume 16, Issue 10
Show Author Affiliations
Kurt R. Denninghoff, The Univ. of Arizona (United States)
Lawrence DeLuca, The Univ. of Arizona (United States)
Katarzyna Sieluzycka, College of Optical Sciences, The Univ. of Arizona (United States)
Jennifer K. Hendryx, College of Optical Sciences, The Univ. of Arizona (United States)
Tyson J. Ririe, College of Optical Sciences, The Univ. of Arizona (United States)
Russell A. Chipman, College of Optical Sciences, The Univ. of Arizona (United States)

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