Share Email Print
cover

Journal of Biomedical Optics • Open Access

Simultaneous optical mapping of transmembrane potential and wall motion in isolated, perfused whole hearts
Author(s): Elliot B. Bourgeois; Andrew D. Bachtel; Jack M. Rogers; Jian Huang; Gregory P. Walcott

Paper Abstract

Optical mapping of cardiac propagation has traditionally been hampered by motion artifact, chiefly due to changes in photodetector-to-tissue registration as the heart moves. We have developed an optical mapping technique to simultaneously record electrical waves and mechanical contraction in isolated hearts. This allows removal of motion artifact from transmembrane potential (Vm) recordings without the use of electromechanical uncoupling agents and allows the interplay of electrical and mechanical events to be studied at the whole organ level. Hearts are stained with the voltage-sensitive dye di-4-ANEPPS and ring-shaped markers are attached to the epicardium. Fluorescence, elicited on alternate frames by 450 and 505 nm light-emitting diodes, is recorded at 700 frames/ per second by a camera fitted with a 605±25 nm emission filter. Marker positions are tracked in software. A signal, consisting of the temporally interlaced 450 and 505 nm fluorescence, is collected from the pixels enclosed by each moving ring. After deinterlacing, the 505 nm signal consists of Vm with motion artifact, while the 450 nm signal is minimally voltage-sensitive and contains primarily artifacts. The ratio of the two signals estimates Vm. Deformation of the tissue enclosed by each set of 3 rings is quantified using homogeneous finite strain.

Paper Details

Date Published: 1 September 2011
PDF: 12 pages
J. Biomed. Opt. 16(9) 096020 doi: 10.1117/1.3630115
Published in: Journal of Biomedical Optics Volume 16, Issue 9
Show Author Affiliations
Elliot B. Bourgeois, The Univ. of Alabama at Birmingham (United States)
Andrew D. Bachtel, The Univ. of Alabama at Birmingham (United States)
Jack M. Rogers, The Univ. of Alabama at Birmingham (United States)
Jian Huang, The Univ. of Alabama at Birmingham (United States)
Gregory P. Walcott, The Univ. of Alabama at Birmingham (United States)


© SPIE. Terms of Use
Back to Top