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Optical Engineering

Tracking of mitochondrial transports using a particle filtering method with a spatial constraint
Author(s): Sungmin Hong; Hackjoon Shim; Yoojin Chung
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Paper Abstract

This paper describes a tracking method to trace the movements of fluorescently labeled mitochondria in time-lapse image sequences. It is based on particle filtering, which is a state-of-the-art tracking method, and is enhanced with a spatial constraint to improve robustness. Since mitochondria move only through axons, the spatial constraint is generated by axon segmentation on a single frame, which is the average of all the frames. The spatial constraint limits the search space of the state vector and, consequently, lowers the chance for the tracking to get lost. Using a background subtraction algorithm, the proposed method is also equipped with automatic detection of starting points, thus minimizing the requirement for user input. Implementation of the proposed method for tracking of fluorescently labeled mitochondria in time-lapse images showed substantially improved robustness and speed compared to a conventional method. With these improvements, this new particle tracking method is expected to increase the throughput of fluorescently labeled mitochondrial transport experiments, which are required for neuroscience research.

Paper Details

Date Published: 1 September 2011
PDF: 10 pages
Opt. Eng. 50(9) 097203 doi: 10.1117/1.3629527
Published in: Optical Engineering Volume 50, Issue 9
Show Author Affiliations
Sungmin Hong, Seoul National Univ. (Korea, Republic of)
Hackjoon Shim, Yonsei Univ. College of Medicine (Korea, Republic of)
Yoojin Chung, Hankuk Univ. of Foreign Studies (Korea, Republic of)

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