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Journal of Biomedical Optics

Spectrally resolved multidepth fluorescence imaging
Author(s): Yuan Luo; Ioannis K. Zervantonakis; Se Baek Oh; Roger D. Kamm; George Barbastathis
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Paper Abstract

We present a multicolor fluorescence imaging modality to visualize in real-time tissue structures emitting multispectral fluorescent light from different focal depths. Each designated spectrum of fluorescent emission from a specific depth within a volumetric tissue is probed by a depth-spectrum selective holographic grating. The grating for each fluorescent color are multiplexed within a volume hologram, which enables simultaneously obtaining multicolored fluorescent information at different depths within a biological tissue sample. We demonstrate the imaging modality's ability to obtain laser-induced multicolored fluorescence images of a biological sample from different depths without scanning. We also experimentally demonstrate that the imaging modality can be simultaneously operated at both fluorescent and bright field modes to provide complementary information of volumetric tissue structures at different depths in real-time.

Paper Details

Date Published: 1 September 2011
PDF: 6 pages
J. Biomed. Opt. 16(9) 096015 doi: 10.1117/1.3626211
Published in: Journal of Biomedical Optics Volume 16, Issue 9
Show Author Affiliations
Yuan Luo, Massachusetts Institute of Technology (United States)
Ioannis K. Zervantonakis, Massachusetts Institute of Technology (United States)
Se Baek Oh, Massachusetts Institute of Technology (United States)
Roger D. Kamm, Massachusetts Institute of Technology (United States)
George Barbastathis, Massachusetts Institute of Technology (United States)

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