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Journal of Biomedical Optics

Lipidots: competitive organic alternative to quantum dots for in vivo fluorescence imaging
Author(s): Julien J. Gravier; Fabrice P. Navarro Y Garcia; Thomas Delmas; Frédérique Mittler; Anne-Claude Couffin; Françoise Vinet; Isabelle Texier-Nogues
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Paper Abstract

The use of fluorescent nanostructures can bring several benefits on the signal to background ratio for in vitro microscopy, in vivo small animal imaging, and image-guided surgery. Fluorescent quantum dots (QDs) display outstanding optical properties, with high brightness and low photobleaching rate. However, because of their toxic element core composition and their potential long term retention in reticulo-endothelial organs such as liver, their in vivo human applications seem compromised. The development of new dye-loaded (DiO, DiI, DiD, DiR, and Indocyanine Green (ICG)) lipid nanoparticles for fluorescence imaging (lipidots) is described here. Lipidot optical properties quantitatively compete with those of commercial QDs (QTracker®705). Multichannel in vivo imaging of lymph nodes in mice is demonstrated for doses as low as 2 pmols of particles. Along with their optical properties, fluorescent lipidots display very low cytotoxicity (IC50 > 75 nM), which make them suitable tools for in vitro, and especially in vivo, fluorescence imaging applications.

Paper Details

Date Published: 1 September 2011
PDF: 11 pages
J. Biomed. Opt. 16(9) 096013 doi: 10.1117/1.3625405
Published in: Journal of Biomedical Optics Volume 16, Issue 9
Show Author Affiliations
Julien J. Gravier, Commissariat à l'Énergie Atomique (France)
Fabrice P. Navarro Y Garcia, Commissariat à l'Énergie Atomique (France)
Thomas Delmas, Commissariat à l'Énergie Atomique (France)
Frédérique Mittler, Commissariat à l'Énergie Atomique (France)
Anne-Claude Couffin, Commissariat à l'Énergie Atomique (France)
Françoise Vinet, Commissariat à l'Énergie Atomique (France)
Isabelle Texier-Nogues, Commissariat à l'Énergie Atomique (France)

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