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Journal of Biomedical Optics

Analyzing near-infrared scattering from human skin to monitor changes in hematocrit
Author(s): Joseph Chaiken; Bin Deng; Jerry Goodisman; George Shaheen; Rebecca J. Bussjaeger
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Paper Abstract

Probing tissue with near-infrared radiation (NIR) simultaneously produces remitted fluorescence and Raman scattering (IE) plus Rayleigh/Mie light scattering (EE) that noninvasively give chemical and physical information about the materials and objects within. We model tissue as a three-phase system: plasma and red blood cell (RBC) phases that are mobile and a static tissue phase. In vivo, any volume of tissue naturally experiences spatial and temporal fluctuations of blood plasma and RBC content. Plasma and RBC fractions may be discriminated from each other on the basis of their physical, chemical, and optical properties. Thus, IE and EE from NIR probing yield information about these fractions. Assuming there is no void volume in viable tissue, or that void volume is constant, changes in plasma and RBC volume fractions may be calculated from simultaneous measurements of the two observables, EE and IE. In a previously published analysis we showed the underlying phenomenology but did not provide an algorithm for calculating volume fractions from experimental data. Now, we present a simple analysis that allows monitoring of fluid fraction and hematocrit (Hct) changes by measuring IE and EE, and apply it to some experimental in vivo measurements.

Paper Details

Date Published: 1 September 2011
PDF: 19 pages
J. Biomed. Opt. 16(9) 097005 doi: 10.1117/1.3625283
Published in: Journal of Biomedical Optics Volume 16, Issue 9
Show Author Affiliations
Joseph Chaiken, Syracuse Univ. (United States)
Bin Deng, Syracuse Univ. (United States)
Jerry Goodisman, Syracuse Univ. (United States)
George Shaheen, LighTouch Medical, Inc. (United States)
Rebecca J. Bussjaeger, LighTouch Medical, Inc. (United States)

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