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Journal of Biomedical Optics

Protoporphyrin IX fluorescence contrast in invasive glioblastomas is linearly correlated with Gd enhanced magnetic resonance image contrast but has higher diagnostic accuracy
Author(s): Kimberley S. Samkoe; Summer L. Gibbs-Strauss; Harold H. Yang; Paul J. Hoopes; Julia A. O'Hara; Brian W. Pogue; S. Khan Hekmatyar; Risto A. Kauppinen
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Paper Abstract

The sensitivity and specificity of in vivo magnetic resonance (MR) imaging is compared with production of protoporphyrin IX (PpIX), determined ex vivo, in a diffusely infiltrating glioma. A human glioma transfected with green fluorescent protein, displaying diffuse, infiltrative growth, was implanted intracranially in athymic nude mice. Image contrast from corresponding regions of interest (ROIs) in in vivo MR and ex vivo fluorescence images was quantified. It was found that all tumor groups had statistically significant PpIX fluorescence contrast and that PpIX contrast demonstrated the best predictive power for tumor presence. Contrast from gadolinium enhanced T1-weighted (T1W+Gd) and absolute T2 images positively predicted the presence of a tumor, confirmed by the GFP positive (GFP+) and hematoxylin and eosin positive (H&E+) ROIs. However, only the absolute T2 images had predictive power from controls in ROIs that were GFP+ but H&E negative. Additionally, PpIX fluorescence and T1W+Gd image contrast were linearly correlated in both the GFP+ (r = 0.79, p<1×10−8) and H&E+ (r = 0.74, p<0.003) ROIs. The trace diffusion images did not have predictive power or significance from controls. This study indicates that gadolinium contrast enhanced MR images can predict the presence of diffuse tumors, but PpIX fluorescence is a better predictor regardless of tumor vascularity.

Paper Details

Date Published: 1 September 2011
PDF: 8 pages
J. Biomed. Opt. 16(9) 096008 doi: 10.1117/1.3622754
Published in: Journal of Biomedical Optics Volume 16, Issue 9
Show Author Affiliations
Kimberley S. Samkoe, Dartmouth Hitchcock Medical Ctr. (United States)
Summer L. Gibbs-Strauss, Dartmouth Hitchcock Medical Ctr. (United States)
Harold H. Yang, Thayer School of Engineering at Dartmouth (United States)
Paul J. Hoopes, Dartmouth Medical School (United States)
Julia A. O'Hara, Dartmouth Medical School (United States)
Brian W. Pogue, Thayer School of Engineering at Dartmouth (United States)
S. Khan Hekmatyar, Dartmouth Medical School (United States)
Risto A. Kauppinen, Dartmouth Medical School (United States)

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