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Journal of Biomedical Optics • Open Access

Rotationally acquired four-dimensional optical coherence tomography of embryonic chick hearts using retrospective gating on the common central A-scan
Author(s): Christoph M. Happel; Talât M. Yelbuz; Jan Thommes; Tobias Ortmaier; Bodo Heimann; Lars Thrane; Jörg Männer

Paper Abstract

We introduce a new method of rotational image acquisition for four-dimensional (4D) optical coherence tomography (OCT) of beating embryonic chick hearts. The rotational axis and the central A-scan of the OCT are identical. An out-of-phase image sequence covering multiple heartbeats is acquired at every angle of an incremental rotation of the deflection mirrors of the OCT system. Image acquisition is accomplished after a rotation of 180°. Comparison of a displayed live M-mode of the central A-scan with a reference M-mode allows instant detection of translational movements of the embryo. For calculation of 4D data sets, we apply an image-based retrospective gating algorithm using the phase information of the common central A-scan present in all acquired images. This leads to cylindrical three-dimensional data sets for every time step of the cardiac cycle that can be used for 4D visualization. We demonstrate this approach and provide a video of a beating Hamburger and Hamilton stage 16 embryonic chick heart generated from a 4D OCT data set using rotational image acquisition.

Paper Details

Date Published: 1 September 2011
PDF: 7 pages
J. Biomed. Opt. 16(9) 096007 doi: 10.1117/1.3622491
Published in: Journal of Biomedical Optics Volume 16, Issue 9
Show Author Affiliations
Christoph M. Happel, Medizinische Hochschule Hannover (Germany)
Talât M. Yelbuz, Medizinische Hochschule Hannover (Germany)
Jan Thommes, Leibniz Univ. Hannover (Germany)
Tobias Ortmaier, Leibniz Univ. Hannover (Germany)
Bodo Heimann, Leibniz Univ. Hannover (Germany)
Lars Thrane, Technical Univ. of Denmark (Denmark)
Jörg Männer, Georg-August-Univ. Göttingen (Germany)


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