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Journal of Biomedical Optics • Open Access

Optical coherence tomography to investigate optical properties of blood during coagulation
Author(s): Xiangqun Xu; Jia Lin; Feifei Fu

Paper Abstract

This study investigates the optical properties of human blood during the coagulation process under statics using optical coherence tomography (OCT). OCT signal slope (OCTSS) and 1/e light penetration depth (d1/e) were obtained from the profiles of reflectance versus depth. Results showed that both OCTSS and d1/e were able to sensitively differentiate various stages of blood properties during coagulating. After 1 h clotting, OCTSS decreased by 47.0%, 15.0%, 13.7%, and 8.5% and d1/e increased by 34.7%, 29.4%, 24.3%, and 22.9% for the blood samples at HCT of 25%, 35%, 45%, and 55%, respectively. The slope of d1/e versus time (Sr, ×10−4 mm/s), associated with clot formation rate decreased from 6.0±0.3, 3.7±0.5 to 2.3±0.4 with the increasing of HCT from 35%, 45%, to 55%. The clotting time (tc) from the d1/e evolution curves was estimated to be 1969±92 s, 375±12 s, 455±11 s, and 865±47 s for the blood of 25%, 35%, 45%, and 55%. This study demonstrates that the parameters (tc and Sr) from the variations in d1/e had better sensitivity and smaller standard deviation. Furthermore, blood hematocrit affecting backscattering properties of blood during coagulation was capable of being discerned by OCT parameters. It is concluded that OCT is a potential technique to quantify and follow the liquid-gel transition of blood during clotting.

Paper Details

Date Published: 1 September 2011
PDF: 8 pages
J. Biomed. Opt. 16(9) 096002 doi: 10.1117/1.3615667
Published in: Journal of Biomedical Optics Volume 16, Issue 9
Show Author Affiliations
Xiangqun Xu, Zhejiang Sci-Tech Univ. (China)
Jia Lin, Zhejiang Sci-Tech Univ. (China)
Feifei Fu, Zhejiang Sci-Tech Univ. (China)

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