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Journal of Biomedical Optics • Open Access

Identification of the direction of the neural network activation with a cellular resolution by fast two-photon imaging
Author(s): Xiuli Liu; Tingwei Quan; Shaoqun Zeng; Xiaohua Lv

Paper Abstract

Spatiotemporal activity patterns in local neural networks are fundamental to understanding how information is processed and stored in brain microcircuits. Currently, imaging techniques are able to map the directional activation of macronetworks across brain areas; however, these strategies still fail to resolve the activation direction for fine microcircuits with cellular spatial resolution. Here, we show the capability to identify the activation direction of a multicell network with a cellular resolution and millisecond precision by using fast two-photon microscopy and cross correlation procedures. As an example, we characterized a directional neuronal network in an epilepsy brain slice to provide different initiation delay among multiple neurons defined at a millisecond scale.

Paper Details

Date Published: 1 August 2011
PDF: 4 pages
J. Biomed. Opt. 16(8) 080506 doi: 10.1117/1.3613918
Published in: Journal of Biomedical Optics Volume 16, Issue 8
Show Author Affiliations
Xiuli Liu, Wuhan National Lab. for Optoelectronics (China)
Tingwei Quan, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Britton Chance Ctr. for Biomedical Photonics (China)
Xiaohua Lv, Huazhong Univ. of Science and Technology (China)

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