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Journal of Biomedical Optics • Open Access

Clinical use of photodynamic antimicrobial chemotherapy for the treatment of deep carious lesions
Author(s): Camila de Almelda B. Guglielmi; Maria A. Luz; Maria R. Lorenzetti Simionato; Karen M. Ramalho; Jose C. P. Imparato; Sérgio L. Pinheiro

Paper Abstract

The purpose of this study was to assess photodynamic antimicrobial chemotherapy (PACT) via irradiation, using a low power laser associated with a photosensitization dye, as an alternative to remove cariogenic microorganisms by drilling. Remaining dentinal samples in deep carious lesions on permanent molars (n = 26) were treated with 0.01% methylene blue dye and irradiated with a low power laser (InGaAIP - indium gallium aluminum phosphide; λ = 660 nm; 100 mW; 320 Jcm−2; 90 s; 9J). Samples of dentin from the pulpal wall region were collected with a micropunch before and immediately after PACT and kept in a transport medium for microbiological analysis. Samples were cultured in plates of Brucella blood agar, Mitis Salivarius Bacitracin agar and Rogosa SL agar to determine the total viable bacteria, mutans streptococci and Lactobacillus spp. counts, respectively. After incubation, colony-forming units were counted and microbial reduction was calculated for each group of bacteria. PACT led to statistically significant reductions in mutans streptococci (1.38 log), Lactobacillus spp. (0.93 log), and total viable bacteria (0.91 log). This therapy may be an appropriate approach for the treatment of deep carious lesions using minimally invasive procedures.

Paper Details

Date Published: 1 August 2011
PDF: 8 pages
J. Biomed. Opt. 16(8) 088003 doi: 10.1117/1.3611009
Published in: Journal of Biomedical Optics Volume 16, Issue 8
Show Author Affiliations
Camila de Almelda B. Guglielmi, Univ. de São Paulo (Brazil)
Maria A. Luz, Univ. de São Paulo (Brazil)
Maria R. Lorenzetti Simionato, Univ. de São Paulo (Brazil)
Karen M. Ramalho, Univ. de São Paulo (Brazil)
Jose C. P. Imparato, Univ. de São Paulo (Brazil)
Sérgio L. Pinheiro, Pontifícia Univ. Católica de São Paulo (Brazil)


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