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Journal of Biomedical Optics • Open Access

Functional laser speckle imaging of cerebral blood flow under hypothermia
Author(s): Minheng Li; Peng Miao; Yisheng Zhu; Shanbao Tong

Paper Abstract

Hypothermia can unintentionally occur in daily life, e.g., in cardiovascular surgery or applied as therapeutics in the neurosciences critical care unit. So far, the temperature-induced spatiotemporal responses of the neural function have not been fully understood. In this study, we investigated the functional change in cerebral blood flow (CBF), accompanied with neuronal activation, by laser speckle imaging (LSI) during hypothermia. Laser speckle images from Sprague-Dawley rats (n = 8, male) were acquired under normothermia (37°C) and moderate hypothermia (32°C). For each animal, 10 trials of electrical hindpaw stimulation were delivered under both temperatures. Using registered laser speckle contrast analysis and temporal clustering analysis (TCA), we found a delayed response peak and a prolonged response window under hypothermia. Hypothermia also decreased the activation area and the amplitude of the peak CBF. The combination of LSI and TCA is a high-resolution functional imaging method to investigate the spatiotemporal neurovascular coupling in both normal and pathological brain functions.

Paper Details

Date Published: 1 August 2011
PDF: 10 pages
J. Biomed. Opt. 16(8) 086011 doi: 10.1117/1.3610995
Published in: Journal of Biomedical Optics Volume 16, Issue 8
Show Author Affiliations
Minheng Li, Shanghai Jiao Tong Univ. (China)
Peng Miao, Shanghai Jiao Tong Univ. (China)
Yisheng Zhu, Shanghai Jiao Tong Univ. (China)
Shanbao Tong, Shanghai Jiao Tong Univ. (China)


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