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Journal of Biomedical Optics • Open Access

Red-shifted fluorescence of sound dental hard tissue
Author(s): Liang Zhang; Leonard Y. Nelson; Eric J. Seibel

Paper Abstract

Autofluorescence spectra were recorded in vitro from dentin, enamel, and whole teeth. The spectra exhibited a broad peak shifted by about 50 to 75 nm from the excitation wavelength and the shape of the spectra remained similar regardless of the excitation wavelength. The maximum of the autofluorescence spectra also exhibited a red-shift that depended upon the laser excitation wavelength. The amplitude of the red-shifted fluorescence spectra produced by 444 and 532 nm excitation lasers were compared to that produced by a 405 nm excitation laser. It was determined that the autofluorescence amplitude was not proportional to the inverse fourth power of the excitation laser wavelength. Therefore, the red-shifted fluorescence is not compatible with the previously proposed mechanism of Raman scattering. Instead, the mechanism giving rise to the laser-induced dental autofluorescence is explained by the red-edge-excitation effect.

Paper Details

Date Published: 1 July 2011
PDF: 6 pages
J. Biomed. Opt. 16(7) 071411 doi: 10.1117/1.3606572
Published in: Journal of Biomedical Optics Volume 16, Issue 7
Show Author Affiliations
Liang Zhang, Univ. of Washington (United States)
Leonard Y. Nelson, Univ. of Washington (United States)
Eric J. Seibel, Univ. of Washington (United States)

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