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Journal of Biomedical Optics • Open Access

Photoacoustic microscopy of tyrosinase reporter gene in vivo
Author(s): Arie Krumholz; Junjie Yao; Lihong V. Wang; Sarah J. vanVickle-Chavez; Timothy Fleming; William E. Gillanders

Paper Abstract

Photoacoustic tomography is a hybrid modality based on optical absorption excitation and ultrasonic detection. It is sensitive to melanin, one of the primary absorbers in skin. For cells that do not naturally contain melanin, melanin production can be induced by introducing the gene for tyrosinase, the primary enzyme responsible for expression of melanin in melanogenic cells. Optical resolution photoacoustic microscopy was used in the ex vivo study reported here, where the signal from transfected cells increased by more than 10 times over wild-type cells. A subsequent in vivo experiment was conducted to demonstrate the capability of photoacoustic microscopy to spectrally differentiate between tyrosinase-catalyzed melanin and various other absorbers in tissue.

Paper Details

Date Published: 1 August 2011
PDF: 4 pages
J. Biomed. Opt. 16(8) 080503 doi: 10.1117/1.3606568
Published in: Journal of Biomedical Optics Volume 16, Issue 8
Show Author Affiliations
Arie Krumholz, Washington Univ. in St. Louis (United States)
Junjie Yao, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)
Sarah J. vanVickle-Chavez, Washington Univ. School of Medicine in St. Louis (United States)
Timothy Fleming, Washington Univ. School of Medicine in St. Louis (United States)
William E. Gillanders, Washington Univ. School of Medicine in St. Louis (United States)


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