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Journal of Biomedical Optics

Two-dimensional and three-dimensional viability measurements of adult stem cells with optical coherence phase microscopy
Author(s): Pierre O. Bagnaninchi; Nicola Drummond; Christina Holmes; Jamal Daoud; Maryam Tabrizian
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Paper Abstract

Cell viability assays are essential tools for cell biology. They assess healthy cells in a sample and enable the quantification of cellular responses to reagents of interest. Noninvasive and label-free assays are desirable in two-dimensional (2D) and three-dimensional (3D) cell culture to facilitate time-course viability studies. Cellular micromotion, emanating from cell to substrate distance variations, has been demonstrated as a marker of cell viability with electric cell-substrate impedance sensing (ECIS). In this study we investigated if optical coherence phase microscopy (OCPM) was able to report phase fluctuations of adult stem cells in 2D and 3D that could be associated with cellular micromotion. An OCPM has been developed around a Thorlabs engine (λo = 930 nm) and integrated in an inverted microscope with a custom scanning head. Human adipose derived stem cells (ADSCs, Invitrogen) were cultured in Mesenpro RS medium and seeded either on ECIS arrays, 2D cell culture dishes, or in 3D highly porous microplotted polymeric scaffolds. ADSC micromotion was confirmed by ECIS analysis. Live and fixed ADSCs were then investigated in 2D and 3D with OCPM. Significant differences were found in phase fluctuations between the different conditions. This study indicated that OCPM could potentially assess cell vitality in 2D and in 3D microstructures.

Paper Details

Date Published: 1 August 2011
PDF: 9 pages
J. Biomed. Opt. 16(8) 086003 doi: 10.1117/1.3606561
Published in: Journal of Biomedical Optics Volume 16, Issue 8
Show Author Affiliations
Pierre O. Bagnaninchi, The Univ. of Edinburgh (United Kingdom)
Nicola Drummond, The Univ. of Edinburgh (United Kingdom)
Christina Holmes, McGill Univ. (Canada)
Jamal Daoud, McGill Univ. (Canada)
Maryam Tabrizian, McGill Univ. (Canada)

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