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Journal of Biomedical Optics • Open Access

Adaptive optics for fluorescence wide-field microscopy using spectrally independent guide star and markers
Author(s): Pierre Vermeulen; Eleonora Muro; Thomas Pons; Vincent Loriette; Alexandra Fragola

Paper Abstract

We describe the implementation and use of an adaptive optics loop in the imaging path of a commercial wide field microscope. We show that it is possible to maintain the optical performances of the original microscope when imaging through aberrant biological samples. The sources used for illuminating the adaptive optics loop are spectrally independent, in excitation and emission, from the sample, so they do not appear in the final image, and their use does not contribute to the sample bleaching. Results are compared with equivalent images obtained with an identical microscope devoid of adaptive optics system.

Paper Details

Date Published: 1 July 2011
PDF: 9 pages
J. Biomed. Opt. 16(7) 076019 doi: 10.1117/1.3603847
Published in: Journal of Biomedical Optics Volume 16, Issue 7
Show Author Affiliations
Pierre Vermeulen, Ecole Supérieure de Physique et de Chimie Industrielles (France)
Eleonora Muro, Ecole Supérieure de Physique et de Chimie Industrielles (France)
Thomas Pons, Ecole Supérieure de Physique et de Chimie Industrielles (France)
Vincent Loriette, Ecole Supérieure de Physique et de Chimie Industrielles (France)
Alexandra Fragola, Ecole Supérieure de Physique et de Chimie Industrielles (France)


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