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Journal of Biomedical Optics

Viability of fibroblasts cultured under nutritional stress irradiated with red laser, infrared laser, and red light-emitting diode
Author(s): Luiz Evaristo Ricci Volpato; Ridrigo Cardoso de Oliveira; Maria Aparecida A Moreira Machado; Mariano Martinez Espinosa; Vanderley Salvador Bagnato
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Paper Abstract

Phototherapy is noninvasive, painless and has no known side effect. However, for its incorporation into clinical practice, more well-designed studies are necessary to define optimal parameters for its application. The viability of fibroblasts cultured under nutritional stress irradiated with either a red laser, an infrared laser, or a red light-emitting diode (LED) was analyzed. Irradiation parameters were: red laser (660 nm, 40 mW, 1 W/cm2), infrared laser (780 nm, 40 mW, 1 W/cm2), and red LED (637 ± 15 nm, 40 mW, 1 W/cm2). All applications were punctual and performed with a spot with 0.4 mm2 of diameter for 4 or 8 s. The Kruskal-Wallis test and analysis of variance of the general linear model (p ≤ 0.05) were used for statistical analysis. After 72 h, phototherapy with low-intensity laser and LED showed no toxicity at the cellular level. It even stimulated methylthiazol tetrazolium assay (MTT) conversion and neutral red uptake of fibroblasts cultured under nutritional stress, especially in the group irradiated with infrared laser (p = 0.004 for MTT conversion and p < 0.001 for neutral red uptake). Considering the parameters and protocol of phototherapy used, it can be concluded that phototherapy stimulated the viability of fibroblasts cultured under nutritional deficit resembling those found in traumatized tissue in which cell viability is reduced.

Paper Details

Date Published: 1 July 2011
PDF: 7 pages
J. Biomed. Opt. 16(7) 075004 doi: 10.1117/1.3602850
Published in: Journal of Biomedical Optics Volume 16, Issue 7
Show Author Affiliations
Luiz Evaristo Ricci Volpato, Univ. of Cuiabá (Brazil)
Ridrigo Cardoso de Oliveira, Univ. de São Paulo (Brazil)
Maria Aparecida A Moreira Machado, Univ. de São Paulo (Brazil)
Mariano Martinez Espinosa, Univ. Federal de Mato Grosso (Brazil)
Vanderley Salvador Bagnato, Univ. de São Paulo (Brazil)

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