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Journal of Biomedical Optics • Open Access

Detecting apoptosis using dynamic light scattering with optical coherence tomography
Author(s): Golnaz Farhat; Gregory J. Czarnota; Michael C. Kolios; Victor X. D. Yang; Adrian Miriampillai

Paper Abstract

A dynamic light scattering technique is implemented using optical coherence tomography (OCT) to measure the change in intracellular motion as cells undergo apoptosis. Acute myeloid leukemia cells were treated with cisplatin and imaged at a frame rate of 166 Hz using a 1300 nm swept-source OCT system at various times over a period of 48 h. Time correlation analysis of the speckle intensities indicated a significant increase in intracellular motion 24 h after treatment. This rise in intracellular motion correlated with histological findings of irregularly shaped and fragmented cells indicative of cell membrane blebbing and fragmentation.

Paper Details

Date Published: 1 July 2011
PDF: 4 pages
J. Biomed. Opt. 16(7) 070505 doi: 10.1117/1.3600770
Published in: Journal of Biomedical Optics Volume 16, Issue 7
Show Author Affiliations
Golnaz Farhat, Univ. of Toronto (Canada)
Gregory J. Czarnota, Univ. of Toronto (Canada)
Michael C. Kolios, Univ. of Toronto (Canada)
Victor X. D. Yang, Ryerson Univ. (Canada)
Adrian Miriampillai, Ryerson Univ. (Canada)

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