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Journal of Biomedical Optics • Open Access

Development of a spatially offset Raman spectroscopy probe for breast tumor surgical margin evaluation
Author(s): Matthew D. Keller; Elizabeth Vargis; Anita Mahadevan-Jansen; Nara de Matos Granja; Robert H. Wilson; Mary-Ann Mycek; Mark C. Kelley

Paper Abstract

The risk of local recurrence for breast cancers is strongly correlated with the presence of a tumor within 1 to 2 mm of the surgical margin on the excised specimen. Previous experimental and theoretical results suggest that spatially offset Raman spectroscopy (SORS) holds much promise for intraoperative margin analysis. Based on simulation predictions for signal-to-noise ratio differences among varying spatial offsets, a SORS probe with multiple source-detector offsets was designed and tested. It was then employed to acquire spectra from 35 frozen-thawed breast tissue samples in vitro. Spectra from each detector ring were averaged to create a composite spectrum with biochemical information covering the entire range from the tissue surface to ∼2 mm below the surface, and a probabilistic classification scheme was used to classify these composite spectra as "negative" or "positive" margins. This discrimination was performed with 95% sensitivity and 100% specificity, or with 100% positive predictive value and 94% negative predictive value.

Paper Details

Date Published: 1 July 2011
PDF: 9 pages
J. Biomed. Opt. 16(7) 077006 doi: 10.1117/1.3600708
Published in: Journal of Biomedical Optics Volume 16, Issue 7
Show Author Affiliations
Matthew D. Keller, Vanderbilt Univ. (United States)
Elizabeth Vargis, Vanderbilt Univ. (United States)
Anita Mahadevan-Jansen, Vanderbilt Univ. (United States)
Nara de Matos Granja, Vanderbilt Univ. Medical Ctr. (United States)
Robert H. Wilson, Univ. of Michigan (United States)
Mary-Ann Mycek, Univ. of Michigan (United States)
Mark C. Kelley, Vanderbilt Univ. Medical Ctr. (United States)

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