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Journal of Biomedical Optics • Open Access

Identification of muscle necrosis in the mdx mouse model of Duchenne muscular dystrophy using three-dimensional optical coherence tomography
Author(s): Blake R. Klyen; David D. Sampson; Thea Shavlakadze; Hannah G. Radley-Crabb; Miranda D. Grounds

Paper Abstract

Three-dimensional optical coherence tomography (3D-OCT) was used to image the structure and pathology of skeletal muscle tissue from the treadmill-exercised mdx mouse model of human Duchenne muscular dystrophy. Optical coherence tomography (OCT) images of excised muscle samples were compared with co-registered hematoxylin and eosin-stained and Evans blue dye fluorescence histology. We show, for the first time, structural 3D-OCT images of skeletal muscle dystropathology well correlated with co-located histology. OCT could identify morphological features of interest and necrotic lesions within the muscle tissue samples based on intrinsic optical contrast. These findings demonstrate the utility of 3D-OCT for the evaluation of small-animal skeletal muscle morphology and pathology, particularly for studies of mouse models of muscular dystrophy.

Paper Details

Date Published: 1 July 2011
PDF: 10 pages
J. Biomed. Opt. 16(7) 076013 doi: 10.1117/1.3598842
Published in: Journal of Biomedical Optics Volume 16, Issue 7
Show Author Affiliations
Blake R. Klyen, The Univ. of Western Australia (Australia)
David D. Sampson, The Univ. of Western Australia (Australia)
Thea Shavlakadze, The Univ. of Western Australia (Australia)
Hannah G. Radley-Crabb, The Univ. of Western Australia (Australia)
Miranda D. Grounds, The Univ. of Western Australia (Australia)


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