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Journal of Biomedical Optics • Open Access

Monte Carlo fluorescence microtomography
Author(s): Alexander X. Cong; Wenxiang Cong; Ge Wang; Matthias C. Hofmann; Yong Xu

Paper Abstract

Fluorescence microscopy allows real-time monitoring of optical molecular probes for disease characterization, drug development, and tissue regeneration. However, when a biological sample is thicker than 1 mm, intense scattering of light would significantly degrade the spatial resolution of fluorescence microscopy. In this paper, we develop a fluorescence microtomography technique that utilizes the Monte Carlo method to image fluorescence reporters in thick biological samples. This approach is based on an l0-regularized tomography model and provides an excellent solution. Our studies on biomimetic tissue scaffolds have demonstrated that the proposed approach is capable of localizing and quantifying the distribution of optical molecular probe accurately and reliably.

Paper Details

Date Published: 1 July 2011
PDF: 4 pages
J. Biomed. Opt. 16(7) 070501 doi: 10.1117/1.3596171
Published in: Journal of Biomedical Optics Volume 16, Issue 7
Show Author Affiliations
Alexander X. Cong, Virginia Polytechnic Institute and State Univ. (United States)
Wenxiang Cong, Virginia Polytechnic Institute and State Univ. (United States)
Ge Wang, Virginia Polytechnic Institute and State Univ. (United States)
Matthias C. Hofmann, Virginia Polytechnic Institute and State Univ. (United States)
Yong Xu, Virginia Polytechnic Institute and State Univ. (United States)


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