Share Email Print
cover

Journal of Biomedical Optics

Label-free oxygen-metabolic photoacoustic microscopy in vivo
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Almost all diseases, especially cancer and diabetes, manifest abnormal oxygen metabolism. Accurately measuring the metabolic rate of oxygen (MRO2) can be helpful for fundamental pathophysiological studies, and even early diagnosis and treatment of disease. Current techniques either lack high resolution or rely on exogenous contrast. Here, we propose label-free metabolic photoacoustic microscopy (mPAM) with small vessel resolution to noninvasively quantify MRO2 in vivo in absolute units. mPAM is the unique modality for simultaneously imaging all five anatomical, chemical, and fluid-dynamic parameters required for such quantification: tissue volume, vessel cross-section, concentration of hemoglobin, oxygen saturation of hemoglobin, and blood flow speed. Hyperthermia, cryotherapy, melanoma, and glioblastoma were longitudinally imaged in vivo. Counterintuitively, increased MRO2 does not necessarily cause hypoxia or increase oxygen extraction. In fact, early-stage cancer was found to be hyperoxic despite hypermetabolism.

Paper Details

Date Published: 1 July 2011
PDF: 12 pages
J. Biomed. Opt. 16(7) 076003 doi: 10.1117/1.3594786
Published in: Journal of Biomedical Optics Volume 16, Issue 7
Show Author Affiliations
Junjie Yao, Washington Univ. in St. Louis (United States)
Konstantin I. Maslov, Washington Univ. in St. Louis (United States)
Yu Zhang, Washington Univ. in St. Louis (United States)
Younan Xia, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


© SPIE. Terms of Use
Back to Top