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Journal of Biomedical Optics

Wavelength-resolved measurements of fluorescence lifetime of indocyanine green
Author(s): Anna Gerega; Norbert Zolek; Tomasz Soltysinski; Daniel Milej; Piotr Sawosz; Beata Toczylowska; Adam Liebert
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Paper Abstract

We study fluorescence lifetime of indocyanine green (ICG) using femtosecond laser and sensitive detection based on time-correlated single-photon counting. A time-resolved multichannel spectral system is constructed and applied for determination of the fluorescence lifetime of the ICG in different solvents. Emission properties of ICG in water, milk, and 1% intralipid solution are investigated. Fluorescence of the fluorophore of different concentrations (in a range of 1.7-160 μM) dissolved in different solutions is excited by femtosecond pulses generated with the use of Ti:Sa laser tuned within the range of 740-790 nm. It is observed that fluorescence lifetime of ICG in water is 0.166 ± 0.02 ns and does not depend on excitation and emission wavelengths. We also show that for the diffusely scattering solvents (milk and intralipid), the lifetime may depend on the dye concentration (especially for large concentrations of ICG). This effect should be taken into account when analyzing changes in the mean time of arrival of fluorescence photons excited in ICG dissolved in such optically turbid media.

Paper Details

Date Published: 1 June 2011
PDF: 10 pages
J. Biomed. Opt. 16(6) 067010 doi: 10.1117/1.3593386
Published in: Journal of Biomedical Optics Volume 16, Issue 6
Show Author Affiliations
Anna Gerega, Institute of Biocybernetics and Biomedical Engineering (Poland)
Norbert Zolek, Institute of Biocybernetics and Biomedical Engineering (Poland)
Tomasz Soltysinski, Institute of Biocybernetics and Biomedical Engineering (Poland)
Daniel Milej, Institute of Biocybernetics and Biomedical Engineering (Poland)
Piotr Sawosz, Institute of Biocybernetics and Biomedical Engineering (Poland)
Beata Toczylowska, Institute of Biocybernetics and Biomedical Engineering (Poland)
Adam Liebert, Institute of Biocybernetics and Biomedical Engineering (Poland)


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