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Journal of Biomedical Optics • Open Access

Characteristics of subgingival calculus detection by multiphoton fluorescence microscopy
Author(s): Oi-Hong Tung; Shyh-Yuan Lee; Yu-Lin Lai; How-Foo Chen

Paper Abstract

Subgingival calculus has been recognized as a major cause of periodontitis, which is one of the main chronic infectious diseases of oral cavities and a principal cause of tooth loss in humans. Bacteria deposited in subgingival calculus or plaque cause gingival inflammation, function deterioration, and then periodontitis. However, subgingival calculus within the periodontal pocket is a complicated and potentially delicate structure to be detected with current dental armamentaria, namely dental x-rays and dental probes. Consequently, complete removal of subgingival calculus remains a challenge to periodontal therapies. In this study, the detection of subgingival calculus employing a multiphoton autofluorescence imaging method was characterized in comparison with a one-photon confocal fluorescence imaging technique. Feasibility of such a system was studied based on fluorescence response of gingiva, healthy teeth, and calculus with and without gingiva covered. The multiphoton fluorescence technology perceived the tissue-covered subgingival calculus that cannot be observed by the one-photon confocal fluorescence method.

Paper Details

Date Published: 1 June 2011
PDF: 8 pages
J. Biomed. Opt. 16(6) 066017 doi: 10.1117/1.3593150
Published in: Journal of Biomedical Optics Volume 16, Issue 6
Show Author Affiliations
Oi-Hong Tung, National Yang-Ming Univ. (Taiwan)
Shyh-Yuan Lee, National Yang-Ming Univ. (Taiwan)
Yu-Lin Lai, Taipei Veterans General Hospital (Taiwan)
How-Foo Chen, National Yang-Ming Univ. (Taiwan)


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