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Journal of Biomedical Optics • Open Access

Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo
Author(s): Jae-Youn Hwang; Daniel L. Farkas; Lali K. Medina-Kauwe; Zeev Gross; Harry B. Gray

Paper Abstract

We report a novel in vivo spectral imaging approach to cancer detection and chemotherapy assessment. We describe and characterize a ratiometric spectral imaging and analysis method and evaluate its performance for tumor detection and delineation by quantitatively monitoring the specific accumulation of targeted gallium corrole (HerGa) into HER2-positive (HER2 +) breast tumors. HerGa temporal accumulation in nude mice bearing HER2 + breast tumors was monitored comparatively by a. this new ratiometric imaging and analysis method; b. established (reflectance and fluorescence) spectral imaging; c. more commonly used fluorescence intensity imaging. We also tested the feasibility of HerGa imaging in vivo using the ratiometric spectral imaging method for tumor detection and delineation. Our results show that the new method not only provides better quantitative information than typical spectral imaging, but also better specificity than standard fluorescence intensity imaging, thus allowing enhanced in vivo outlining of tumors and dynamic, quantitative monitoring of targeted chemotherapy agent accumulation into them.

Paper Details

Date Published: 1 June 2011
PDF: 7 pages
J. Biomed. Opt. 16(6) 066007 doi: 10.1117/1.3589299
Published in: Journal of Biomedical Optics Volume 16, Issue 6
Show Author Affiliations
Jae-Youn Hwang, Cedars-Sinai Medical Ctr. (United States)
Daniel L. Farkas, Cedars-Sinai Medical Ctr. (United States)
Lali K. Medina-Kauwe, Cedars-Sinai Medical Ctr. (United States)
Zeev Gross, Technion-Israel Institute of Technology (Israel)
Harry B. Gray, California Institute of Technology (United States)

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