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Journal of Biomedical Optics • Open Access

Chemical changes demonstrated in cartilage by synchrotron infrared microspectroscopy in an antibody-induced murine model of rheumatoid arthritis
Author(s): Allyson M. Croxford; Merrill J. Rowley; Don McNaughton; Kutty Selva Nandakumar; Rikard Holmdahl; Mark J. Tobin

Paper Abstract

Collagen antibody-induced arthritis develops in mice following passive transfer of monoclonal antibodies (mAbs) to type II collagen (CII) and is attributed to effects of proinflammatory immune complexes, but transferred mAbs may react directly and damagingly with CII. To determine whether such mAbs cause cartilage damage in vivo in the absence of inflammation, mice lacking complement factor 5 that do not develop joint inflammation were injected intravenously with two arthritogenic mAbs to CII, M2139 and CIIC1. Paws were collected at day 3, decalcified, paraffin embedded, and 5-µm sections were examined using standard histology and synchrotron Fourier-transform infrared microspectroscopy (FTIRM). None of the mice injected with mAb showed visual or histological evidence of inflammation but there were histological changes in the articular cartilage including loss of proteoglycan and altered chondrocyte morphology. Findings using FTIRM at high lateral resolution revealed loss of collagen and the appearance of a new peak at 1635 cm-1 at the surface of the cartilage interpreted as cellular activation. Thus, we demonstrate the utility of synchrotron FTIRM for examining chemical changes in diseased cartilage at the microscopic level and establish that arthritogenic mAbs to CII do cause cartilage damage in vivo in the absence of inflammation.

Paper Details

Date Published: 1 June 2011
PDF: 10 pages
J. Biomed. Opt. 16(6) 066004 doi: 10.1117/1.3585680
Published in: Journal of Biomedical Optics Volume 16, Issue 6
Show Author Affiliations
Allyson M. Croxford, Monash Univ. (Australia)
Merrill J. Rowley, Monash Univ. (Australia)
Don McNaughton, Monash Univ. (Australia)
Kutty Selva Nandakumar, Karolinska Institutet (Sweden)
Rikard Holmdahl, Karolinska Institutet (Sweden)
Mark J. Tobin, Australian Synchrotron Co. Ltd. (Australia)

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