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Journal of Biomedical Optics • Open Access

Mitochondrial function and tissue vitality: bench-to-bedside real-time optical monitoring system
Author(s): Avraham Mayevsky; Eliyahu Pewzner; Assaf Deutsch; Raphael Walden; Eitan Heldenberg; Jacob Lavee; Salis Tager; Erez Kachel; Ehud Raanani; Sergey Preisman; Violete Glauber; Eran Segal

Paper Abstract

Background: The involvement of mitochondria in pathological states, such as neurodegenerative diseases, sepsis, stroke, and cancer, are well documented. Monitoring of nicotinamide adenine dinucleotide (NADH) fluorescence in vivo as an intracellular oxygen indicator was established in 1950 to 1970 by Britton Chance and collaborators. We use a multiparametric monitoring system enabling assessment of tissue vitality. In order to use this technology in clinical practice, the commercial developed device, the CritiView (CRV), is tested in animal models as well as in patients. Methods and Results: The new CRV enables the optical monitoring of four different parameters, representing the energy balance of various tissues in vivo. Mitochondrial NADH is measured by surface fluorometry/reflectometry. In addition, tissue microcirculatory blood flow, tissue reflectance and oxygenation are measured as well. The device is tested both in vitro and in vivo in a small animal model and in preliminary clinical trials in patients undergoing vascular or open heart surgery. In patients, the monitoring is started immediately after the insertion of a three-way Foley catheter (urine collection) to the patient and is stopped when the patient is discharged from the operating room. The results show that monitoring the urethral wall vitality provides information in correlation to the surgical procedure performed.

Paper Details

Date Published: 1 June 2011
PDF: 22 pages
J. Biomed. Opt. 16(6) 067004 doi: 10.1117/1.3585674
Published in: Journal of Biomedical Optics Volume 16, Issue 6
Show Author Affiliations
Avraham Mayevsky, Bar-Ilan Univ. (Israel)
Eliyahu Pewzner, CritiSense Ltd. (Israel)
Assaf Deutsch, CritiSense Ltd. (Israel)
Raphael Walden, Sheba Medical Ctr. (Israel)
Eitan Heldenberg, Sheba Medical Ctr. (Israel)
Jacob Lavee, Sheba Medical Ctr. (Israel)
Salis Tager, Sheba Medical Ctr. (Israel)
Erez Kachel, Sheba Medical Ctr. (Israel)
Ehud Raanani, Sheba Medical Ctr. (Israel)
Sergey Preisman, Sheba Medical Ctr. (Israel)
Violete Glauber, Sheba Medical Ctr. (Israel)
Eran Segal, Assuta Medical Ctrs. (Israel)

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