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Journal of Biomedical Optics • Open Access

Automatic cytometric device using multiple wavelength excitations
Author(s): Nelly Rongeat; Sylvain Ledroit; Laurence Chauvet; Didier Cremien; Alexandra Urankar; Philippe Nerin; Vincent Couderc

Paper Abstract

Precise identification of eosinophils, basophils, and specific subpopulations of blood cells (B lymphocytes) in an unconventional automatic hematology analyzer is demonstrated. Our specific apparatus mixes two excitation radiations by means of an acousto-optics tunable filter to properly control fluorescence emission of phycoerythrin cyanin 5 (PC5) conjugated to antibodies (anti-CD20 or anti-CRTH2) and Thiazole Orange. This way our analyzer combining techniques of hematology analysis and flow cytometry based on multiple fluorescence detection, drastically improves the signal to noise ratio and decreases the spectral overlaps impact coming from multiple fluorescence emissions.

Paper Details

Date Published: 1 May 2011
PDF: 8 pages
J. Biomed. Opt. 16(5) 057006 doi: 10.1117/1.3582162
Published in: Journal of Biomedical Optics Volume 16, Issue 5
Show Author Affiliations
Nelly Rongeat, HORIBA ABX (France)
Sylvain Ledroit, HORIBA ABX (France)
Laurence Chauvet, HORIBA ABX (France)
Didier Cremien, HORIBA ABX (France)
Alexandra Urankar, HORIBA ABX (France)
Philippe Nerin, HORIBA ABX (France)
Vincent Couderc, Univ. de Limoges (France)

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