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Journal of Biomedical Optics

Enhanced two-channel nonlinear imaging by a highly polarized supercontinuum light source generated from a nonlinear photonic crystal fiber with two zero-dispersion wavelengths
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Paper Abstract

Real-time monitoring the variation of chlorophyll distributions and cellular structures in leaves during plant growth provides important information for understanding the physiological statuses of plants. Two-photon-excited autofluorescence imaging and second harmonic generation imaging of leaves can be used for monitoring the nature intrinsic fluorophores distribution and cellular structures of leaves by the use of the near-infrared region of light which has minimal light absorption by endogenous molecules and thus increases tissue penetration. However, the two-photon absorption peak of intrinsic fluorophores of a ficus benjamina leaf is 50 nm away from the second harmonic generation excitation wavelength, which cannot be effectively excited by a femtosecond laser beam with one central wavelength. This paper shows that a highly polarized supercontinuum light generated from a birefringent nonlinear photonic crystal fiber with two zero-dispersion wavelengths can effectively excite two-photon autofluorescence as well as second harmonic generation signals for simultaneously monitoring intrinsic fluorophore distributions and non-centrosymmetric structures of leaves.

Paper Details

Date Published: 1 May 2011
PDF: 5 pages
J. Biomed. Opt. 16(5) 056010 doi: 10.1117/1.3580279
Published in: Journal of Biomedical Optics Volume 16, Issue 5
Show Author Affiliations
Wei Tao, Swinburne Univ. of Technology (Australia)
Hong Chun Bao, Swinburne Univ. of Technology (Australia)
Min Gu, Swinburne Univ. of Technology (Australia)

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