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Journal of Biomedical Optics

Spectroscopic signature of mouse embryonic stem cell-derived hepatocytes using synchrotron Fourier transform infrared microspectroscopy
Author(s): Kanjana Thumanu; Waraporn Tanthanuch; Danna Ye; Anawat Sangmalee; Rangsun Parnpai; Chanchao Lorthongpanich; Philip Heraud
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Paper Abstract

Stem cell-based therapy for liver regeneration has been proposed to overcome the persistent shortage in the supply of suitable donor organs. A requirement for this to succeed is to find a rapid method to detect functional hepatocytes, differentiated from embryonic stem cells. We propose Fourier transform infrared (FTIR) microspectroscopy as a versatile method to identify the early and last stages of the differentiation process leading to the formation of hepatocytes. Using synchrotron-FTIR microspectroscopy, the means of identifying hepatocytes at the single-cell level is possible and explored. Principal component analysis and subsequent partial least-squares (PLS) discriminant analysis is applied to distinguish endoderm induction from hepatic progenitor cells and matured hepatocyte-like cells. The data are well modeled by PLS with endoderm induction, hepatic progenitor cells, and mature hepatocyte-like cells able to be discriminated with very high sensitivity and specificity. This method provides a practical tool to monitor endoderm induction and has the potential to be applied for quality control of cell differentiation leading to hepatocyte formation.

Paper Details

Date Published: 1 May 2011
PDF: 9 pages
J. Biomed. Opt. 16(5) 057005 doi: 10.1117/1.3580253
Published in: Journal of Biomedical Optics Volume 16, Issue 5
Show Author Affiliations
Kanjana Thumanu, Synchrotron Light Research Institute (Thailand)
Waraporn Tanthanuch, Synchrotron Light Research Institute (Thailand)
Danna Ye, Suranaree Univ. of Technology (Thailand)
Anawat Sangmalee, Suranaree Univ. of Technology (Thailand)
Rangsun Parnpai, Suranaree Univ. of Technology (Thailand)
Chanchao Lorthongpanich, A*STAR Institute of Medical Biology (Singapore)
Philip Heraud, Monash Univ. (Australia)

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